Modifications of plasma proteome in healthy humans fed o a coenzymen Q-10 supplemented diet

Comunicaciones a congreso

Long‑term efect of a dietary intervention with two‑healthy dietary approaches on food intake and nutrient density in coronary patients: results from the CORDIOPREV trial

Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. N.C.-I. is a recipient of the Juan de la Cierva Formación Programme. E.M.Y-S is a recipient of the Nicolás Monardes Programme (C1-0005–2019, Servicio Andaluz de Salud, Junta de Andalucía, Spain). A.P.A-L is supported by a Río-Hortega Programme (Instituto de Salud Carlos III). The CORDIOPREV study is supported by the Fundacion Patrimonio Comunal Olivarero. The sponsor was not involved in the design or carrying out the study, and its participation was limited to funding and providing the olive oil used in the study. CORDIOPREV trial also received additional funding from CITOLIVA, CEAS, Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud and Ministerio de Medio Ambiente, Medio Rural y Marino and the Spanish Government. It was also partly supported by research grants from the Ministerio de Ciencia e Innovacion (AGL2009-122270 to J L-M, FIS PI10/01041 to P. P.-M, FIS PI13/00023 to J. D.-L); Ministerio de Economia y Competitividad (AGL2012/39615 to J L-M); Consejeria de Salud, Junta de Andalucia (PI0193/09 to J. L.-M, PI-0252/09 to J. D.-L, and PI-0058/10 to P. P.-M, PI-0206–2013 to A. G.-R); Proyecto de Excelencia, Consejería de Economía, Innovación, Ciencia y Empleo (CVI-7450 to J. L.-M).Background Cardiovascular disease (CVD) is the leading cause of disease burden in the world by non-communicable diseases. Nutritional interventions promoting high-quality dietary patterns with low caloric intake value and high nutrient density (ND) could be linked to a better control of CVD risk and recurrence of coronary disease. This study aims to assess the efects of a dietary intervention based on MedDiet or Low-Fat dietary intervention over changes in ND and food intake after 1 and 7 years of follow-up of the CORDIOPREV study. Methods We prospectively analyzed the results of the 802 coronary patients randomized to two healthy dietary patterns (MedDiet=425, Low-Fat Diet=377) who completed the 7 years of follow-up and had all the dietary data need. Dietary intake information obtained from a validated 137-item Food Frequency Questionnaire was used to calculate 1- and 7-year changes in dietary intake and ND (measured as nutrient intake per 1000 kcal). T test was used to ascertain diferences in food intake and ND between groups across follow-up time. Within-subject (dietary allocation group) diferences were analyzed with ANOVA repeated measures. Results From baseline to 7 years of follow-up, signifcant increases of vegetables, fruits, and whole cereals within groups (p<0.001) was found. We found a higher increase in dietary intake of certain food groups with MedDiet in comparison with Low-Fat Diet for vegetables (46.1 g/day vs. 18.1 g/day, p<00.1), fruits (121.3 g/day vs. 72.9 g/day), legumes (4.3 g/day vs. 0.16 g/day) and nuts (7.3 g/day vs. − 3.7 g/day). There was a decrease in energy intake over time in both groups, slightly higher in Low-Fat Diet compared to MedDiet group (− 427.6 kcal/day vs. − 279.8 kcal/day at 1st year, and − 544.6 kcal/ day vs. − 215.3 kcal/day after 7 years of follow-up). ND of all the nutrients increased within group across follow-up time, except for Saturated Fatty Acids (SFA), cholesterol and sodium (p<0.001). Conclusions A comprehensive dietary intervention improved quality of diet, reducing total energy intake and increasing the intake of healthy food groups and overall ND after 1 year and maintaining this trend after 7 years of follow-up. Our results reinforce the idea of the participation in trials, enhance nutrition literacy and produces better nutritional outcomes in adult patients with established CVD. Clinical trial registry The trial was registered in 2009 at ClinicalTrials.gov (number NCT00924937)CITOLIVACentro de Excelencia en Investigacion sobre Aceite de Oliva y SaludConsejeria de Agricultura y PescaConsejeria de InnovacionConsejeria de SaludConsejeria de Salud, Junta de Andalucia PI-0058/10, PI-0206–2013, PI-0252/09, PI0193/09Consejería de Economía, Innovación, Ciencia y Empleo CVI-7450Diputaciones de Jaen y CordobaFundacion Patrimonio Comunal OlivareroMedio Rural y MarinoMinisterio de Ciencia e Innovacion AGL2009-122270, FIS PI10/01041, FIS PI13/00023Spanish GovernmentCenter for East Asian Studies, Stanford UniversityMinisterio de Economía y Competitividad AGL2012/39615Instituto de Salud Carlos IIIMinistério do Meio AmbienteJunta de Andalucí

Renal clear cell sarcoma in the pediatric age: differential diagnosis and management

Renal clear cell sarcoma is the second most frequent malignant renal tumor in pediatric age, after Wilms' tumor. It is a difficult to diagnose neoplasm, with nonspecific clinical presentation as palpable abdominal mass, abdominal pain and hypertension and hematuria. The imaging characteristics of this lesion are non-specific, so its diagnosis is made by anatomopathological study of the tumor. Its importance revolves around the diagnostic difficulty, since this tumor presents great histologic variability and few adequate immunohistochemical markers. Treatment includes neoadjuvant and post-surgical chemotherapy, with a prognosis that improves if the disease is diagnosed early

Ternary Quantum Dots in Chemical Analysis. Synthesis and Detection Mechanisms

Ternary quantum dots (QDs) are novel nanomaterials that can be used in chemical analysis due their unique physicochemical and spectroscopic properties. These properties are size-dependent and can be adjusted in the synthetic protocol modifying the reaction medium, time, source of heat, and the ligand used for stabilization. In the last decade, several spectroscopic methods have been developed for the analysis of organic and inorganic analytes in biological, drug, environmental, and food samples, in which different sensing schemes have been applied using ternary quantum dots. This review addresses the different synthetic approaches of ternary quantum dots, the sensing mechanisms involved in the analyte detection, and the predominant areas in which these nanomaterials are usedThe authors give thanks to the CONACYT support for the grant number 771019S

Planning and management of ecological tourism of the Middle Eastern Atlas in Morocco

La diversificación de los productos turísticos en Marruecos se revela como uno de los principios básicos para el impulso y el crecimiento económico del país, según el documento Estrategia del desarrollo turístico. Visión 2.010. Actualmente Marruecos presenta una oferta polarizada en el turismo convencional (sol y playa, desierto, ciudades imperiales). El turismo ecológico es una de las posibles alternativas dada la diversidad de los paisajes y de los recursos ecológicos del país. En este artículo se presentan las potencialidades de cara al ecoturismo del Medio Atlas oriental al sur de Taza, señalando fortalezas y oportunidades, así como detectando los principales puntos débiles. Se trata de una zona con interesantes valores ecológicos y naturales que, debidamente planificada y gestionada, puede ofertarse para el disfrute de los visitantes, la conservación de las riquezas naturales y el crecimiento económico de la región. Para ello se realiza una serie de propuestas de planificación y gestión basadas en el estudio de las comunidades rurales allí asentadas, en el análisis directo de los recursos ecológicos y en los nuevos escenarios de cooperación y planificación socio-económica de Marruecos.La diversificaciò dels productes turístics al Marroc es revela com un dels principis bàsics per a l'impuls i el creixement econòmic del país, segons el document Estrategia del desarrollo turístico.Visión 2.010. Actualment, el Marroc presenta una oferta polaritzada en el turisme convencional (sol i platja, desert, ciutats imperials). El turisme ecològic és una de les possibles alternatives donada la diversitat dels paisatges i dels recursos ecològics del país. En aquest article espresenten les potencialitats de cara a l'ecoturisme de l'Atlas Mitjà oriental al sud de Taza, assenyalant-ne fortaleses i oportunitats, aixó com detectant-ne els principals punts dèbils. Es tracta d'una zona amb interessants valors ecològics i naturals que, degudament planificada i gestionada, pot oferir-se per a gaudi dels visitants, la conservació de les riqueses naturals i el creixement econòmic de la regió. Per aixó es realitzen una sèrie de propostes de planificació i gestió basades en l'estudi de les comunitats rurals que s'hi troben, en l'anàlisi directa dels recursos ecològics i en els nous escenaris de cooperació i planificació socioeconòmica del Marroc.The diversification of tourist products in Morocco is revealed as one of the basic principles for the impulse and economic growth of the country, according to the document Strategy of the tourist development. Vision 2.010, since nowadays it shows a supply focused on conventional tourism (sun and beach, desert, imperial cities). The Ecological tourism is one of the possible alternatives that Morocco can supply, given the diversity of its landscapes and their ecological resources. In this article the potentialities for the ecotourism of the Middle Eastern Atlas to the south of Taza are considered, indicating the strengths and the opportunities, as well as detecting the main weak points. It is a zone with interesting ecological and natural values that, properly planned and managed, can be offered for the benefit of the visitors, the conservation of the natural wealth and the economic growth of the region. For this purpose a series of proposals of planning and management based on the study of their rural communities is made, in the direct analysis of the ecological resources and in the new scenes of cooperation and socio-economic planning of Morocco.Agencia Española de Cooperación Internacional (AECI

Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

A sensitive electrochemical detection method for Salmonella spp. was described, based on the use of Hechtia argentea lectin immobilised on a screen-printed gold electrode. The lectin was extracted from Hechtia argentea, a plant belonging to the Bromeliaceae family. The lectin with molecular weight near 27.4 kDa showed selectivity towards D-mannose, contained on the lipopolysaccharide cell wall of Salmonella spp. Carbohydrate selectivity of the lectin was measured as a change in impedance with respect to concentration. The binding of the bacteria to the biosensor surface increased impedance with increasing concentrations of Salmonella spp., achieving a linear range of detection of 15–2.57 × 107 CFU mL−1, with a limit of detection of 5 CFU mL−1. Increases in impedance were measured using electrochemical impedance spectroscopy and analysed using Nyquist plots. The biosensor was applied in analysis of hen egg samples, and the results were consistent with those obtained using the official analysis methodologyS

Magnetic molecularly imprinted stirring bar for isolation of patulin using grafting technique

The 19th International Electronic Conference on Synthetic Organic Chemistry session Polymer and Supramolecular ChemistryMycotoxins are low-molecular-weight natural products with great structural diversity produced as secondary metabolites by fungi. One of the principal toxic fungal metabolites is patulin (PAT), produced by over 30 genera of mold including species as Penicillium expansum or Penicillium griseofulvum, and normally related to vegetable-based products and fruit, mainly apple. These mold grow easily in damaged fruit or in derived-product as juices if storage conditions are deficient. Some of the most serious effects of PAT ingestion are agitation, convulsions, edema, ulceration intestinal, inflammation and vomiting. Thus, European Regulation 1881/2006 established a maximum content of 10 ppb in infant fruit juices, 50 ppb for fruit juices in adults and 25 ppb in fruit-derived products. Nowadays, the official analytical method for food adopted by AOAC International is HPLC with UV detection, using clean-up with ethyl acetate and sodium carbonate. However, the diverse drawbacks of this method (poor stability of PAT under alkaline extraction, poor resolution between PAT and co-extracted hydroxymethylfurfural) have originated interest in alternative options, such as LC methods coupled to mass spectrometry. In the last years, purification with molecularly imprinted polymers (MIP) started to be used, and are becoming promising materials for extracting different analytes present in food. Mycotoxins are too toxic or too expensive to be used as template molecules in MIP preparation. Template “bleeding” may be an additional problem, especially when dealing with very low detection levels. In the present work, a rapid and selective method based on magnetic molecularly imprinted stir-bar (MMIB) extraction has been developed for the isolation of PAT. A structural analogue, 2-oxindole, was used as dummy template. The polymer was grafted to the silanized glass surface of the stir ba